Product Name :
Phospho-mTOR (S2448) Mouse Monoclonal Antibody [A5D5]
Predicted band size :
289 kDa
Observed band size :
289 kDa
Synonyms:
dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody FK506 binding protein 12 rapamycin associated protein 1 antibody FK506 binding protein 12 rapamycin associated protein 2 antibody FK506 binding protein 12 rapamycin complex associated protein 1 antibody FK506-binding protein 12-rapamycin complex-associated protein 1 antibody FKBP rapamycin associated protein antibody FKBP12 rapamycin complex associated protein antibody FKBP12-rapamycin complex-associated protein 1 antibody FKBP12-rapamycin complex-associated protein antibody FLJ44809 antibody 展开
Function :
The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to Rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.
Antibody Type:
Mouse Monoclonal Antibody
Immunogen :
Synthetic peptide within human mTOR aa 2430-2480.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P
Coupling :
unconjugated
Clone number:
A5D5
RRID:
AB_3071710
Form :
Liquid
Concentration :
2ug/ul
Storage instructions:
Store at +4℃ after thawing.Price of 2-(tert-Butyl)thiazole-5-carboxylic acid Aliquot store at -20℃.4-Chloro-2-ethynylaniline web Avoid repeated freeze / thaw cycles.PMID:33543447
Storage buffer solution:
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG1
Purification method :
Protein G affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 289 kDa
Positive control :
HeLa cell lysate, HeLa starved overnight then treated with 200nM PMA for 4 hours cell lysate, NIH/3T3 cell lysate treated with serum-starvation overnight, NIH/3T3 cell lysate treated with Insulin (100nM) for 10 minutes after serum-starvation overnight, human liver tissue.